Overview
I am a Senior Cytometry Scientist with the Sydney Cytometry Core Research Facility, an Honorary Research Fellow with the Infection, Immunity, and Inflammation theme and Sydney Infectious Disease Institute, and co-leader of the Single Cell and Spatial Biology Research Node with the Charles Perkins Centre at the University of Sydney. In these roles I specialise in the use of cutting edge high-dimensional cytometry, single-cell, and spatial multiomics technologies for interrogating cellular systems. My work involves the development of new computational and experimental methods to better understand immune development and pathology across the human lifespan, with a particular interest in the pathogenesis of severe respiratory and neuroinvasive infectious diseases.
I received my Ph.D in Medicine (Immunology) from the University of Sydney, where I used Australia’s first mass cytometry system to discover a role for inflammatory haematopoiesis in the pathogenesis of viral encephalitis. Subsequently I served as a high-dimensional cytometry specialist with the Sydney Cytometry Core Research Facility, and held visiting scientist positions with Stanford University and the Francis Crick Institute in London. Across these roles I developed and implemented multiple new techniques for cytometry, single-cell, and spatial analysis, helping to discover key determinants of host protection and pathology in important diseases including COVID-19.
My work
- Through the Sydney Cytometry Core Research Facility, I provide a range of services in assay design, sample preparation, sample acquisition, and data analysis for high-dimensional cytometry, single-cell, and spatial multiomics technologies.
- Details of these services can be found through the Sydney Cytometry website.
- My work involves developing new laboratory and computational methods for cellular and spatial analysis. We apply these methods to study the development of the immune system over the human lifetime in health and disease.
- Details of this work can be found through the Immune Dynamics website.
Interests
Resources
Publications
Selected publications. Full list on Google Scholar
Training & Experience
Achievements
- Established the University of Sydney’s first single-cell and spatial multiomics service lab as part of the Sydney Cytometry Core Research Facility
- Introduced new methods for data integration to create a high-dimensional cytometry atlas of the developing human system in peripheral blood.
Roles:
- Visiting Scientist, Francis Crick Institute, London (2024)
- Honorary Research Fellow, Infectious Disease and Immunology
- Co-lead, Single Cell and Spatial Biology Research Node
Achievements:
- Management of high-dimensional flow and mass cytometry (CyTOF)
- Introduced Australia’s first Imaging Mass Cytometer, and the University of Sydney’s first spectral cytometry systems
- Aided important discoveries in the immune response against COVID-19
- Introduced the Spectre computational toolkit for high-dimensional cytometry analysis
- Co-founded the Single Cell and Spatial Biology Research Node of the Charles Perkins Centre
- Published the first 27-colour flow cytometry panels
Roles:
- Honorary Research Fellow, Infectious Disease and Immunology (2021-present)
- Co-lead, Single Cell and Spatial Biology Research Node (2021-present)
- Systems Cytometry and NSW representative, Systems Immunology specialist interest group of the Australia and New Zealand Society for Immunology (2020 – 2023)
- International Society for the Advancement of Cytometry (ISAC) Marylou Ingram Scholar (2017-2022)
Achievements:
- Thesis: “Mobilisation of the murine haematopoietic system in the bone marrow during viral encephalitis”
- Mapping inflammatory changes to haematopoiesis during viral encephalitis in Australia’s first CyTOF experiments
Roles:
- Visiting Scientist, Stanford University (2014) supported by a Sydney Medical School travelling fellowship
- Majors: Immunobiology, Virology
- Honours: Cell Pathology